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| Registros recuperados: 67 | |
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| Pommepuy, Monique; Dupray, Elisabeth; Guillaud, Jean-francois; Derrien, A; L'Yavanc, Jacky; Cormier, M. |
| The behaviour of fecal bacteria was investigated in a turbid estuary which is also a shellfishing area. Sediments are polluted by sewage by the way of settlement, fecal bacteria mixed with estuarine water being able to survive a very long time (T90 are about several days). By measuring the increase of salt tolerance of the strains grown in natural estuarine organic matter, it was demonstrated that Escherichia coli is able to support the salinity of coastal water. Moreover, because light penetration is obstructed by suspended matter, the solar bactericidal effect is very low. A close relationship between T90 and light intensity received by the bacteria is found. These mechanisms can explain the low die-off rate found in turbid areas. |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00267/37860/35941.pdf |
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| Le Guyader, Soizick; Haugarreau, Larissa; Miossec, Laurence; Dubois, Eric; Pommepuy, Monique. |
| The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or... |
| Tipo: Text |
Palavras-chave: Molecular technique; Seasonal variation; Human pathology; Viral contamination; Shellfish; Enteric virus. |
| Ano: 2000 |
URL: https://archimer.ifremer.fr/doc/00507/61879/65953.pdf |
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| Le Saux, Jean-claude; Serais, Ophelie; Krol, Joanna; Parnaudeau, Sylvain; Salvagnac, P.; Delmas, G.; Cicchelero, V.; Claudet, J.; Pothier, P.; Balay, K.; Fiandrino, Annie; Pommepuy, Monique; Le Guyader, Soizick. |
| Infectious diseases linked to the consumption of raw shellfish have long been identified. Over the past century, various strategies have been set up in shellfish growing areas throughout the world to guarantee the sanitary quality of shellfish and to protect consumers. However despite sanitary improvements, human enteric viruses - especially Hepatitis A virus and norovirus– have been found to be associated with shellfish outbreaks. A recent example demonstrated the impact of storm events. Following heavy rain and sewage overflow, shellfish beds were contaminated and the shellfish from them were marketed after depuration. However, since viruses persist longer than fecal contamination indicator bacteria, several clusters of gastroenteritis cases were... |
| Tipo: Text |
Palavras-chave: Virus; Shellfish; Contamination events. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17736/15257.pdf |
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| Pommepuy, Monique; Le Saux, Jean-claude; Hervio Heath, Dominique; Le Guyader, Soizick. |
| Infectious diseases linked to the consumption of raw shellfish like oysters, mussels, cockles and clams, have long been identified. Bacterial diseases such as cholera and typhoid fever were the first to be suspected of being linked to consumption of contaminated shellfish (Butt et al., 2004). During the past century, various strategies have been established in shellfish growing areas throughout the world to assure the sanitary quality of shellfish. More recently, despite sanitary surveys, Vibrio parahaemolyticus, Vibrio vulnificus and enteric viruses - especially Hepatitis A virus (HAV) and norovirus (NoV) – were found to be associated in outbreaks of human illness. |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00015/12607/9485.pdf |
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| Loisy, Fabienne; Atmar, R; Le Saux, Jean-claude; Cohen, J; Caprais, Marie-paule; Pommepuy, Monique; Le Guyader, Soizick. |
| Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log(10) of VLPs was depurated after 1 week in warm seawater (22 degrees C). Phage MS2 was depurated more rapidly (about 2 log(10) in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10(10) and 10(9) particles per g of pancreatic tissue)... |
| Tipo: Text |
Palavras-chave: Seawater; Viral diseases; Bioaccumulation; Depuration; Contamination; Model; Pancreas; Regression analysis; Shellfish; Phages; Virus. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1233.pdf |
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| Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M.. |
| An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to... |
| Tipo: Text |
Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf |
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| Zidane, Mohamed; Le Guyader, Soizick; Pommepuy, Monique. |
| During the winter 2000/2001, several outbreaks associated with the consumption of shellfish were declared in France. Following these events, a close cooperation was organized between administrations and laboratories (DGAL, DPMA, InVS, AFSSA, Ifremer, CHU-Dijon and CNC) to gather competences and to manage the situation. This report synthesizes the information collected during these outbreaks. From the outset, the epidemiologic investigations directed the research towards viral contamination and shellfish as contamination source. Several outbreaks were investigated. Oysters and stools samples from ill patients were analysed by RT-PCR for viruses (enterovirus, rotavirus, norovirus and astrovirus). The genic amplification was done by using the same primers in... |
| Tipo: Text |
Palavras-chave: Épidémie; Gastro-entérites; Huîtres; Contamination virale; RT-PCR; Norovirus; TIAC. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00068/17941/15476.pdf |
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| Bougeard, Morgane; Le Saux, Jean-claude; Perenne, Nicolas; Le Guyader, Soizick; Pommepuy, Monique. |
| During rainstorms, watersheds can introduce large amounts of faecal pollution into the rivers and sea, leading to shellfish contamination. In this study, we assessed Escherichia coli fluxes from a catchment, and their impact on estuarine water quality, using two assembled models. For the catchment, the agro-hydrological model SWAT was implemented integrating land uses, soil, topography, rainfall and other climatic data on Daoulas watershed (France). Initially, the SWAT model was calibrated and validated for river flow rates, and results were found satisfactory. Subsequently, different faecal contamination sources were integrated into the model: point sources (WWTP discharges into rivers) and non-point sources (manure spreading on fields). During rainfall... |
| Tipo: Text |
Palavras-chave: Modeling; SWAT; MARS; E. coli; Shellfish; Catchment; Estuary. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17748/15270.pdf |
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| Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique. |
| Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between... |
| Tipo: Text |
Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf |
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| Miossec, Laurence; Le Guyader, Francoise; Haeghebaert, S.; Gasnier, Ph.; Bellier, J.y.; Vaillant, V.; Camus, P.; Pommepuy, Monique; Abou-saleh, M.j.; Clavelin, Ph.; Bobo, J.p.; Masson, D.; Desenclos, J.c.. |
| Tipo: Text |
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| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00001/11244/7780.pdf |
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| Troussellier, Marc; Bonnefont, Jean-luc; Courties, Claude; Derrien, Annick; Dupray, Elizabeth; Gauthier, Michel; Gourmelon, Michele; Joux, Fabien; Lebaron, Philippe; Martin, Yvan; Pommepuy, Monique. |
| The effects of different environmental factors (nutrient deprivation, hyperosmotic shock, exposure to light) on enteric bacteria which have been transferred into the marine environment, have been studied experimentally (microcosms) by considering demographic, physiological and genetic responses in Escherichia coli or Salmonella typhimurium populations. Short-term experiments (less than or equal to 48 h) showed that nutrient deprivation induced limited changes in measured bacteriological variables, but when combined with hyperosmotic shock, it results in an energy charge decrease and inactivation of membrane transport. Light exposure mainly affects the colony-forming capacity of bacterial populations. Combining different stress factors confirmed the rapid... |
| Tipo: Text |
Palavras-chave: Genetics; Cellular states; Physiology; Seawater; Enteric bacteria; Génétique; Etats cellulaires; Physiologie; Eau de mer; Entérobactéries. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-845.pdf |
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| Gourmelon, Michele; Montet, Mp; Loaec, Solen; Le Mennec, Cecile; Pommepuy, Monique; Vernoy-rozand, C. |
| La qualité sanitaire du milieu marin côtier est influencée par la présence de rejets urbains ou de rejets diffus d'origine agricole. Ces effluents sont riches en bactéries ou virus d'origine fécale, dont certains ont été mis en cause dans des pathologies humaines (Beeching, 1997 ; Feldhusen, 2000 ; Lipp et Rose, 1997 ; Yam et al., 1999). Parmi les microorganismes pouvant présenter un danger pour l'homme, certains, pathogènes émergents ces dernières années, n'ont été que récemment recherchés dans l'environnement : on peut citer les Cryptosporidium et les Giardia pour les parasites, les norovirus pour les virus et les Escherichia coli vérotoxiques (VTEC) pour les bactéries. Ces derniers ne sont connus que depuis 1982, date à laquelle E. coli 0157 : H7 a été... |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00106/21697/19276.pdf |
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| Wieliczko, Etienne; Gourmelon, Michele; Dupray, Elisabeth; Derrien, A.; Caprais, Marie-paule; Pommepuy, Monique. |
| D'un point de vue purement écologique, le milieu marin est particulièrement agressif pour les bactéries d'origine entérique. Jusqu'à la fin des années soixante dix, il était généralement admis que ces bactéries pathogènes étaient détruites en quelques heures dans l'eau de mer, ou selon l'espèce en quelques jours. On parlait alors du "pouvoir auto-épurateur" de l'eau de mer. Depuis des travaux plus récents ont démontré la persistance de microorganismes dans les zones littorales. En fait, la survie en mer des bactéries d'origine fécale dans le milieu marin dépend de deux grands facteurs : la qualité de l'eau ou du sédiment permettant aux bactéries de mettre en oeuvre plus ou moins facilement des mécanismes de "résistance", et leur historique avant leur rejet... |
| Tipo: Text |
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| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00105/21630/19210.pdf |
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| Pommepuy, Monique. |
| Les zones marines littorales reçoivent des rejets d'eaux usées d'origine urbaine et agricole qui contiennent de très grandes quantités de micro-organismes dont certains sont pathogènes pour l'homme ; dans environnements hostiles, la dispersion et les conditions vie défavorables font que ces bactéries et virus disparaissent rapidement ou évoluent vers un état non cultivable. Cependant, certains milieux - sédiments, eaux turbides ou eutrophisées présentent favorables à la survie bactéries et à la protection des particules virales, Les travaux réalisés ces dernières ont apporté la preuve que bactéries sont capables de survivre dans l'environnement, d'adapter leur métabolisme aux conditions naturelles et sur très longues périodes ; dans ces conditions évoluent... |
| Tipo: Text |
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| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00040/15158/12494.pdf |
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| Caprais, Marie-paule; Le Mennec, Cecile; Pommepuy, Monique; Le Saux, Jean-claude; Gourmelon, Michele. |
| In Europe, shellfish harvesting areas are classified according European Directive EC N°854/2004, using classic faecal indicator levels (Escherichia coli). A better indicator is needed, however, because shellfish may contain other human pathogens. Other indicator organisms have therefore been evaluated and, among these, coliphages meet most of the requirements to serve as indicators and models for enteroviruses. In order to evaluate the capacity of coliphages as indicators, a total of 764 shellfish were sampled in 2004 from 70 shellfish harvesting areas monitored by the Ifremer microbiological surveillance network (REMI, http://www.ifremer.fr/envlit/), and tested for F-specific RNA bacteriophages (FRNAPH). Two species of bivalve mollusc were collected... |
| Tipo: Text |
Palavras-chave: F-specific RNA bacteriophage; Shellfish; Faecal pollution; Discrimination. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17749/15271.pdf |
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| Registros recuperados: 67 | |
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